dialysate contains unphysiological levels of glucose as the osmotic agent. This constant exposure to hyperosmolar and low pH dialysis solution, with high glucose concentration causes additional injury to the peritoneal mesothelium, already damaged by the uremic internal millieu

Investigating peritoneal membrane alterations caused by peritoneal dialysis fluid during peritoneal dialysis in humans is still intriguing. That is why the study was aimed to provide rabbit peritoneal tissue samples suitable for histological analyses using a modified nonuremic infusion model of peritoneal dialysis on rabbit. A double-lumen central venous catheter, surgically placed in the peritoneal cavity of adult Chinchilla rabbit, was used for daily instillations of peritoneal dialysate. Peritoneal tissue samples were obtained during the catheter placement and removal and analysed by light and transmission electron microscopy. The results of histological examinations showed that this modified non-uremic rabbit model of peritoneal dialysis is suitable for obtaining peritoneal tissue samples for light and transmission electron microscopy examination, and can be used to analyze the effects of different dialysis solutions on rabbit peritoneal membrane.